Review

human lymph node metastatic melanoma cell line sk mel 28 (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC human lymph node metastatic melanoma cell line sk mel 28
Human Lymph Node Metastatic Melanoma Cell Line Sk Mel 28, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metastatic+human+melanoma+cell+line/SK-MEL-28/10__3390_slash_antiox15060740-69-11-19
Average 99 stars, based on 2212 article reviews

human lymph node metastatic melanoma cell line sk mel 28 - by Bioz Stars, 2026-10

99/100 stars

Images

other:

Article Title: Lymphatic trafficking kinetics and near-infrared imaging using star polymer architectures with controlled anionic character
Article Snippet: The lymphatically metastatic human melanoma cell line, A2058, was purchased from ATCC and was maintained in low sodium bicarbonate DMEM (ATCC) supplemented with 1% l -Glutamine and 10% bovine growth serum in a 5% CO 2 atmosphere.

Article Title: Drug and imaging agent delivery compositions and methods
Article Snippet: The lymphatically metastatic human melanoma cell line, A2058, was purchased from ATCC, and was maintained in low sodium bicarbonate DMEM (ATCC) supplemented with 1% L-Glutamine, and 10% bovine growth serum in 5% CO2 atmosphere.

Article Title: Formins and Arp2/3 Reciprocally Regulate Contact Guidance on Aligned Collagen Fibrils
Article Snippet: Collagen fibril samples were first blocked with blocking buffer (0.02 g ml −1 BSA prepared in 10% TBS containing 0.01% Tween-20) for 20 minutes and then incubated with 1:100 collagen antibody (polyclonal rabbit, Thermo fisher, Bio-Rad-2150-1908) for 24 hours.

Article Title: In vitro effects of undifferentiated callus extracts from Plantago major L, Rhodiola rosea L and Silybum marianum L in normal and malignant human skin cells.
Article Snippet: The metastatic human melanoma cell line (A2058) and the epidermal human carcinoma cell line (A431) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Article Title: Lymphatic trafficking kinetics and near-infrared imaging using star polymer architectures with controlled anionic character
Article Snippet: The molecular weights and polydispersity index (PDI) of the protected star polymer, poly-(1,2:3,4- Di - O -isopropylidene-6- O -methacryloyl-α- D -galactopyranose), and deprotected star polymer, poly-(6- O -methacryloyl- D -galactose) 2 , were determined by size exclusion chromatography (SEC) (Shimadzu 2010CHT) using a TSK gel multipore Hx-M column coupled with a refractive index detector (Shimadzu RID-10A), and using 0.8 ml/min of DMF containing 10-mM LiCl as the mobile phase.

Article Title: In vitro effects of undifferentiated callus extracts from Plantago major L, Rhodiola rosea L and Silybum marianum L in normal and malignant human skin cells
Article Snippet: The metastatic human melanoma cell line (A2058) and the epidermal human carcinoma cell line (A431) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Multiple Displacement Amplification:

Article Title: FHOD3 and DIAPH3 control cell migration and differentially shift the balance of parallel and perpendicular stress fibers
Article Snippet: .. A human mammary carcinoma cell line (MDA-MB-231, ATCC, Manassas, VA), an immortalized human foreskin fibroblast cell line (HFF, ATCC), an immortalized human keratinocyte cell line (HaCaT, kind gift from Dr. Torsten Wittmann), a metastatic human melanoma cell line (WM-266-4, ATCC) and an immortalized monocytic cell line (THP-1, ATCC) were used in this study. .. MDA-MB-231, HaCaT and WMM266-4 cells were cultured in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich, St. Louis, MO), supplemented with 10% fetal bovine serum (Gibco).

Image Search Results

SLC45A2 knockdown inhibits cell migration and clonogenicity in uveal melanoma C918 and MuM2B cells. (A, B) qRT-PCR and Western blotting to evaluate the level of SLC45A2 mRNA and protein levels 48 h after transfection. All siRNA sequences could result in significant decrease in SLC45A2 mRNA and protein levels expression (P<0.001). (C) Transwell assay. The migration and invasion capacity of C918 and MuM2B cells decreased significantly after SLC45A2 knockdown. (D) Scratch-wound healing assay. A significantly slower wound healing rate was observed in cells with a decreased expression of SLC45A2 gene. All data were presented as the means ± SD of three independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001).

Journal: Frontiers in Immunology

Article Title: Single-cell profiling deciphering cholesterol metabolism dysregulation in metastatic uveal melanoma and implicating SLC45A2 in its prognosis

doi: 10.3389/fimmu.2025.1660268

Figure Lengend Snippet: SLC45A2 knockdown inhibits cell migration and clonogenicity in uveal melanoma C918 and MuM2B cells. (A, B) qRT-PCR and Western blotting to evaluate the level of SLC45A2 mRNA and protein levels 48 h after transfection. All siRNA sequences could result in significant decrease in SLC45A2 mRNA and protein levels expression (P<0.001). (C) Transwell assay. The migration and invasion capacity of C918 and MuM2B cells decreased significantly after SLC45A2 knockdown. (D) Scratch-wound healing assay. A significantly slower wound healing rate was observed in cells with a decreased expression of SLC45A2 gene. All data were presented as the means ± SD of three independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: Briefly, Human metastatic uveal melanoma cell line C918 (CL-0264 Procell Inc., Wuhan, China) and MuM2B (FH-1158 Fuheng Biotechnology Inc., Shanghai, China) was cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum.

Techniques: Knockdown, Migration, Quantitative RT-PCR, Western Blot, Transfection, Expressing, Transwell Assay, Wound Healing Assay

(A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in MeWo-GFP or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant metastatic A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.

Journal: bioRxiv

Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

doi: 10.1101/2025.09.06.674616

Figure Lengend Snippet: (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in MeWo-GFP or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant metastatic A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.

Article Snippet: Human metastatic melanoma cell lines MeWo (ATCC #HTB-65) , A375M , and A375P-GFP (ATCC #CRL-3224), as well as HEK-293FT cells (Thermo Fisher), were maintained in complete RPMI-1640 medium (MeWo, A375M; Gibco, #21875-034) or in complete DMEM (A375P, HEK-293FT; Gibco, # 41965-039), supplemented with 10% fetal bovine serum (Eurobio, #CVFSVF00-0U) and 1% penicillin/streptomycin (Sigma, #P0781).

Techniques: Xenograft Assay, Migration, Transfection, Microscopy, Knockdown, Control, Injection

(A) Protein expression levels of the EMT-TFs SNAIL2, SNAIL1, TWIST, ZEB2 and ZEB1 across different melanoma cell lines. (B) mRNA expression of EMT-TFs in MeWo cells assessed by qPCR 48h after transfection with siCtrl or siPFKFB4. Statistical analysis was performed using an unpaired t-test. (C, D) Normalized protein expression of EMT-TFs in MeWo (C) or A375M (D) cells, 48h after transfection with siCtrl or siPFKFB4. Each dot represents one biological replicate with paired values connected by lines. Statistical analysis was performed using a paired t- test. n.s, not significant (P > 0.05); *P < 0.05; **P < 0.01.

Journal: bioRxiv

Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

doi: 10.1101/2025.09.06.674616

Figure Lengend Snippet: (A) Protein expression levels of the EMT-TFs SNAIL2, SNAIL1, TWIST, ZEB2 and ZEB1 across different melanoma cell lines. (B) mRNA expression of EMT-TFs in MeWo cells assessed by qPCR 48h after transfection with siCtrl or siPFKFB4. Statistical analysis was performed using an unpaired t-test. (C, D) Normalized protein expression of EMT-TFs in MeWo (C) or A375M (D) cells, 48h after transfection with siCtrl or siPFKFB4. Each dot represents one biological replicate with paired values connected by lines. Statistical analysis was performed using a paired t- test. n.s, not significant (P > 0.05); *P < 0.05; **P < 0.01.

Techniques: Expressing, Transfection

(A) Mean migration velocity of MeWo cells 48 h after transfection with siCtrl or siRNA targeting SNAIL2 (siSNAIL2). Kernel density plots illustrate the distribution of migration speeds in control versus SNAIL2 knockdown groups. (B,C) Expression of PFKFB4 and SNAIL2 proteins (B) and mean migration velocity (C) of MeWo cells transfected with siCtrl or siRNA targeting PFKFB4 (siPFKFB4), combined with empty pcDNA vector or a plasmid expressing Xenopus laevis SNAIL2 (xSNAIL2). Kernel density plots show the distribution of migration speeds across groups. xSNAIL2 failed to rescue the migration defect induced by PFKFB4 knockdown. (D) Mean migration velocity of MeWo cells after siCtrl or siPFKFB4 transfection, combined with empty pcDNA vector or a plasmid expressing Xenopus laevis PFKFB4 (xPFKFB4). Kernel density plots show the distribution of migration speeds. xPFKFB4 was able to rescue the migration defect caused by PFKFB4 knockdown. (E, F) Protein expression levels of PFKFB4 and SNAIL2 (E) and quantification from four biological replicates (F) in MeWo cells transfected with siCtrl or siPFKFB4, combined with empty pcDNA or xPFKFB4 plasmid. xPFKFB4 failed to rescue the decrease in SNAIL2 protein levels caused by PFKFB4 knockdown. In panels A , C , and D , bar plots represent mean ±SEM and were analyzed using an unpaired t- test. (F) Each dot represents one biological replicate, with paired values connected by lines; significance was assessed using a paired t- test. n.s., not significant (P > 0.05); *P < 0.05.

Journal: bioRxiv

Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

doi: 10.1101/2025.09.06.674616

Figure Lengend Snippet: (A) Mean migration velocity of MeWo cells 48 h after transfection with siCtrl or siRNA targeting SNAIL2 (siSNAIL2). Kernel density plots illustrate the distribution of migration speeds in control versus SNAIL2 knockdown groups. (B,C) Expression of PFKFB4 and SNAIL2 proteins (B) and mean migration velocity (C) of MeWo cells transfected with siCtrl or siRNA targeting PFKFB4 (siPFKFB4), combined with empty pcDNA vector or a plasmid expressing Xenopus laevis SNAIL2 (xSNAIL2). Kernel density plots show the distribution of migration speeds across groups. xSNAIL2 failed to rescue the migration defect induced by PFKFB4 knockdown. (D) Mean migration velocity of MeWo cells after siCtrl or siPFKFB4 transfection, combined with empty pcDNA vector or a plasmid expressing Xenopus laevis PFKFB4 (xPFKFB4). Kernel density plots show the distribution of migration speeds. xPFKFB4 was able to rescue the migration defect caused by PFKFB4 knockdown. (E, F) Protein expression levels of PFKFB4 and SNAIL2 (E) and quantification from four biological replicates (F) in MeWo cells transfected with siCtrl or siPFKFB4, combined with empty pcDNA or xPFKFB4 plasmid. xPFKFB4 failed to rescue the decrease in SNAIL2 protein levels caused by PFKFB4 knockdown. In panels A , C , and D , bar plots represent mean ±SEM and were analyzed using an unpaired t- test. (F) Each dot represents one biological replicate, with paired values connected by lines; significance was assessed using a paired t- test. n.s., not significant (P > 0.05); *P < 0.05.

Techniques: Migration, Transfection, Control, Knockdown, Expressing, Plasmid Preparation

Cell viability of MM28 human metastatic uveal melanoma cells ( a ) treated with 0–10 µM of quercetin ( b ) and 0.1–10 µM of doxorubicin, which serves as a positive control. The absorbance of treated samples was normalized to untreated control supplemented with DMSO at 0 h. One-way ANOVA test was applied for statistical analysis (** p < 0.005, *** p < 0.0005, **** p < 0.0001).

Journal: Life

Article Title: Low-Dose Quercetin Dephosphorylates AKT and Suppresses Proteins Related to Migration in Human Metastatic Uveal Melanoma Cells

doi: 10.3390/life15060979

Figure Lengend Snippet: Cell viability of MM28 human metastatic uveal melanoma cells ( a ) treated with 0–10 µM of quercetin ( b ) and 0.1–10 µM of doxorubicin, which serves as a positive control. The absorbance of treated samples was normalized to untreated control supplemented with DMSO at 0 h. One-way ANOVA test was applied for statistical analysis (** p < 0.005, *** p < 0.0005, **** p < 0.0001).

Article Snippet: The MM28 human metastatic uveal melanoma cell line was purchased from ATCC (CRL-3295).

Techniques: Positive Control, Control

mRNA expression levels of proliferation markers in MM28 cells after 1 µM quercetin treatment for a 72 h period. Total RNA was isolated, and then 100 ng of cDNA was used for investigation of the gene expression of AKT, PI3K, PTEN, and Maspin, using GAPDH as a housekeeping gene. The expression levels in samples treated by quercetin were normalized to control samples (untreated). Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was applied for statistical analysis (** p < 0.005, **** p < 0.0001). After the treatment, there was no significant change in PTEN ( p = 0.7711), whereas AKT ( p = 0.0047) and PI3K ( p = 0.0011) significantly decreased, and Maspin ( p < 0.0001) showed a significant elevation.

Journal: Life

Article Title: Low-Dose Quercetin Dephosphorylates AKT and Suppresses Proteins Related to Migration in Human Metastatic Uveal Melanoma Cells

doi: 10.3390/life15060979

Figure Lengend Snippet: mRNA expression levels of proliferation markers in MM28 cells after 1 µM quercetin treatment for a 72 h period. Total RNA was isolated, and then 100 ng of cDNA was used for investigation of the gene expression of AKT, PI3K, PTEN, and Maspin, using GAPDH as a housekeeping gene. The expression levels in samples treated by quercetin were normalized to control samples (untreated). Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was applied for statistical analysis (** p < 0.005, **** p < 0.0001). After the treatment, there was no significant change in PTEN ( p = 0.7711), whereas AKT ( p = 0.0047) and PI3K ( p = 0.0011) significantly decreased, and Maspin ( p < 0.0001) showed a significant elevation.

Article Snippet: The MM28 human metastatic uveal melanoma cell line was purchased from ATCC (CRL-3295).

Techniques: Expressing, Isolation, Gene Expression, Control

mRNA expression levels of migration markers in MM28 cells after 1 µM quercetin treatment for a 72 h period. Total RNA was isolated, then 100 ng of cDNA was used for the analysis of the expression of MMP2 and MMP9 genes, using GAPDH as a housekeeping gene. The expression levels of quercetin-treated samples were normalized to untreated control samples. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was used for statistical analysis (**** p < 0.0001). After the treatment, there were opposite significant changes in MMP2 ( p < 0.0001) and MMP9 ( p < 0.0001).

Journal: Life

Article Title: Low-Dose Quercetin Dephosphorylates AKT and Suppresses Proteins Related to Migration in Human Metastatic Uveal Melanoma Cells

doi: 10.3390/life15060979

Figure Lengend Snippet: mRNA expression levels of migration markers in MM28 cells after 1 µM quercetin treatment for a 72 h period. Total RNA was isolated, then 100 ng of cDNA was used for the analysis of the expression of MMP2 and MMP9 genes, using GAPDH as a housekeeping gene. The expression levels of quercetin-treated samples were normalized to untreated control samples. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was used for statistical analysis (**** p < 0.0001). After the treatment, there were opposite significant changes in MMP2 ( p < 0.0001) and MMP9 ( p < 0.0001).

Article Snippet: The MM28 human metastatic uveal melanoma cell line was purchased from ATCC (CRL-3295).

Techniques: Expressing, Migration, Isolation, Control

Western blot analysis of proliferation markers in MM28 cells after 1 µM of quercetin treatment for a 72 h period. The protein expression levels of quercetin-treated samples were normalized to untreated control samples. ( a ) Western blot images of AKT, pAKT, PI3K, PTEN, and HPRT proteins. ( b ) Band intensities of AKT, pAKT, PI3K, and PTEN protein expressions. The protein expressions were normalized to the HPRT housekeeping protein. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was used for statistical analysis (*** p < 0.0005, **** p < 0.0001). After the treatment, there were no significant changes in AKT ( p = 0.0576) and PTEN ( p = 0.3481), while pAKT ( p = 0.0002) and PI3K ( p < 0.0001) showed opposite significant changes.

Journal: Life

Article Title: Low-Dose Quercetin Dephosphorylates AKT and Suppresses Proteins Related to Migration in Human Metastatic Uveal Melanoma Cells

doi: 10.3390/life15060979

Figure Lengend Snippet: Western blot analysis of proliferation markers in MM28 cells after 1 µM of quercetin treatment for a 72 h period. The protein expression levels of quercetin-treated samples were normalized to untreated control samples. ( a ) Western blot images of AKT, pAKT, PI3K, PTEN, and HPRT proteins. ( b ) Band intensities of AKT, pAKT, PI3K, and PTEN protein expressions. The protein expressions were normalized to the HPRT housekeeping protein. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was used for statistical analysis (*** p < 0.0005, **** p < 0.0001). After the treatment, there were no significant changes in AKT ( p = 0.0576) and PTEN ( p = 0.3481), while pAKT ( p = 0.0002) and PI3K ( p < 0.0001) showed opposite significant changes.

Article Snippet: The MM28 human metastatic uveal melanoma cell line was purchased from ATCC (CRL-3295).

Techniques: Western Blot, Expressing, Control

Changes in protein expression level of NF-κB p65 in MM28 cells by Western blot analysis after 1 µM quercetin treatment for a 72 h period. The expression levels of quercetin-treated samples were normalized to control samples (untreated). ( a ) Western blot images of NF-κB p65 and HPRT proteins. ( b ) Band intensities of NF-κB p65 protein expression. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was used for statistical analysis (** p < 0.005). After the treatment, there was a significant decrease in NF-κB p65 ( p = 0.0043).

Journal: Life

Article Title: Low-Dose Quercetin Dephosphorylates AKT and Suppresses Proteins Related to Migration in Human Metastatic Uveal Melanoma Cells

doi: 10.3390/life15060979

Figure Lengend Snippet: Changes in protein expression level of NF-κB p65 in MM28 cells by Western blot analysis after 1 µM quercetin treatment for a 72 h period. The expression levels of quercetin-treated samples were normalized to control samples (untreated). ( a ) Western blot images of NF-κB p65 and HPRT proteins. ( b ) Band intensities of NF-κB p65 protein expression. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was used for statistical analysis (** p < 0.005). After the treatment, there was a significant decrease in NF-κB p65 ( p = 0.0043).

Article Snippet: The MM28 human metastatic uveal melanoma cell line was purchased from ATCC (CRL-3295).

Techniques: Expressing, Western Blot, Control

Western blot analysis of protein expression levels of MMP2, MMP8, and MMP9 in MM28 cells after 1 µM of quercetin treatment for a 72 h period. The expression levels of quercetin-treated samples were normalized to untreated control samples. ( a ) Western blot images of MMP2, MMP8, MMP9, and HPRT proteins. The PVDF membrane probed with an antibody for pAKT was stripped and reprobed with an antibody for MMP8; using the same HPRT band for densitometric analysis, samples were running under identical loading conditions. ( b ) Band intensities of MMP2, MMP8, and MMP9 protein expressions. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was used for statistical analysis (** p < 0.005). After the treatment, there were no significant changes in MMP2 ( p = 0.9473) and MMP9 ( p = 0.1273), while MMP8 was greatly suppressed ( p = 0.0027).

Journal: Life

Article Title: Low-Dose Quercetin Dephosphorylates AKT and Suppresses Proteins Related to Migration in Human Metastatic Uveal Melanoma Cells

doi: 10.3390/life15060979

Figure Lengend Snippet: Western blot analysis of protein expression levels of MMP2, MMP8, and MMP9 in MM28 cells after 1 µM of quercetin treatment for a 72 h period. The expression levels of quercetin-treated samples were normalized to untreated control samples. ( a ) Western blot images of MMP2, MMP8, MMP9, and HPRT proteins. The PVDF membrane probed with an antibody for pAKT was stripped and reprobed with an antibody for MMP8; using the same HPRT band for densitometric analysis, samples were running under identical loading conditions. ( b ) Band intensities of MMP2, MMP8, and MMP9 protein expressions. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was used for statistical analysis (** p < 0.005). After the treatment, there were no significant changes in MMP2 ( p = 0.9473) and MMP9 ( p = 0.1273), while MMP8 was greatly suppressed ( p = 0.0027).

Article Snippet: The MM28 human metastatic uveal melanoma cell line was purchased from ATCC (CRL-3295).

Techniques: Western Blot, Expressing, Control, Membrane

Proteome Profiler Human XL Oncology Array analysis of Cathepsin B, Endoglin/CD105, Enolase 2, HO-1/HMOX-1, ICAM-1/CD54, progranulin, and vimentin protein expression levels in MM28 cells after 1 µM of quercetin treatment for a 72 h period. ( a ) Oncology array pictures of control and 1 µM quercetin treated samples of MM28 cells. ( b ) Mean pixel density of Cathepsin B, Endoglin/CD105, Enolase 2, HO-1/HMOX-1, ICAM-1/CD54, progranulin, and vimentin after treatment with 1 µM quercetin. The expression levels of quercetin-treated samples were normalized to untreated control samples. Data represent mean values ± S.D. Student’s t -test was used for statistical analysis (* p < 0.05, ** p < 0.005). After the treatment, there was no significant change in Enolase 2 ( p = 0.0539), while Cathepsin B ( p = 0.0371), Endoglin/CD105 ( p = 0.0016), HO-1 ( p = 0.0016), ICAM-1 ( p = 0.0414), progranulin ( p = 0.0083), and vimentin ( p = 0.0028) showed a significant reduction.

Journal: Life

Article Title: Low-Dose Quercetin Dephosphorylates AKT and Suppresses Proteins Related to Migration in Human Metastatic Uveal Melanoma Cells

doi: 10.3390/life15060979

Figure Lengend Snippet: Proteome Profiler Human XL Oncology Array analysis of Cathepsin B, Endoglin/CD105, Enolase 2, HO-1/HMOX-1, ICAM-1/CD54, progranulin, and vimentin protein expression levels in MM28 cells after 1 µM of quercetin treatment for a 72 h period. ( a ) Oncology array pictures of control and 1 µM quercetin treated samples of MM28 cells. ( b ) Mean pixel density of Cathepsin B, Endoglin/CD105, Enolase 2, HO-1/HMOX-1, ICAM-1/CD54, progranulin, and vimentin after treatment with 1 µM quercetin. The expression levels of quercetin-treated samples were normalized to untreated control samples. Data represent mean values ± S.D. Student’s t -test was used for statistical analysis (* p < 0.05, ** p < 0.005). After the treatment, there was no significant change in Enolase 2 ( p = 0.0539), while Cathepsin B ( p = 0.0371), Endoglin/CD105 ( p = 0.0016), HO-1 ( p = 0.0016), ICAM-1 ( p = 0.0414), progranulin ( p = 0.0083), and vimentin ( p = 0.0028) showed a significant reduction.

Article Snippet: The MM28 human metastatic uveal melanoma cell line was purchased from ATCC (CRL-3295).

Techniques: Expressing, Control

mRNA expression levels of Endoglin, HO-1, and vimentin in MM28 cells after 1 µM quercetin treatment for a 72 h period. Total RNA was isolated, then 100 ng of cDNA was used for the evaluation, using GAPDH as a housekeeping gene. The expression levels of quercetin-treated samples were normalized to untreated control samples. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was applied for statistical analysis (*** p < 0.0005, **** p < 0.0001). After the treatment, there were significant reductions in Endoglin ( p < 0.0001), HO-1 ( p < 0.0001), and vimentin ( p = 0.0003).

Journal: Life

Article Title: Low-Dose Quercetin Dephosphorylates AKT and Suppresses Proteins Related to Migration in Human Metastatic Uveal Melanoma Cells

doi: 10.3390/life15060979

Figure Lengend Snippet: mRNA expression levels of Endoglin, HO-1, and vimentin in MM28 cells after 1 µM quercetin treatment for a 72 h period. Total RNA was isolated, then 100 ng of cDNA was used for the evaluation, using GAPDH as a housekeeping gene. The expression levels of quercetin-treated samples were normalized to untreated control samples. Treatments were performed in three independent experiments and were expressed as the mean ± S.D. Student’s t -test was applied for statistical analysis (*** p < 0.0005, **** p < 0.0001). After the treatment, there were significant reductions in Endoglin ( p < 0.0001), HO-1 ( p < 0.0001), and vimentin ( p = 0.0003).

Article Snippet: The MM28 human metastatic uveal melanoma cell line was purchased from ATCC (CRL-3295).

Techniques: Expressing, Isolation, Control

Identification of differentially expressed genes based on ImmuneScore and StromalScore. ( A-C ) Overall survival analysis of high and low Immune/Stromal/ESTIMATE Scores in patients with metastatic melanoma using the ESTIMATE algorithm. ( D , E ) Heatmap depicting the top 100 DEmRNAs with the most significant p-values between high and low Immune/Stromal Scores (|log Fold change|>1.9, p < 0.001). Blue represents downregulation, while red represents upregulation. ( F ) Venn diagram showing the upregulated and downregulated genes commonly intersecting between Stromal and Immune Scores, with green indicating immune-related DEmRNAs and purple indicating stromal-related DEmRNAs. ( G ) Construction of a protein-protein interaction network for overlapping genes using the STRING online tool (confidence score > 0.900) and Cytoscape software. ( H ) The top 15 genes ranked by nodes in the PPI network are shown in the graph. ( I ) Univariate Cox analysis was conducted on genes with a node count of 15 or more in the PPI network to identify differentially expressed genes significantly associated with prognosis

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Identification of differentially expressed genes based on ImmuneScore and StromalScore. ( A-C ) Overall survival analysis of high and low Immune/Stromal/ESTIMATE Scores in patients with metastatic melanoma using the ESTIMATE algorithm. ( D , E ) Heatmap depicting the top 100 DEmRNAs with the most significant p-values between high and low Immune/Stromal Scores (|log Fold change|>1.9, p < 0.001). Blue represents downregulation, while red represents upregulation. ( F ) Venn diagram showing the upregulated and downregulated genes commonly intersecting between Stromal and Immune Scores, with green indicating immune-related DEmRNAs and purple indicating stromal-related DEmRNAs. ( G ) Construction of a protein-protein interaction network for overlapping genes using the STRING online tool (confidence score > 0.900) and Cytoscape software. ( H ) The top 15 genes ranked by nodes in the PPI network are shown in the graph. ( I ) Univariate Cox analysis was conducted on genes with a node count of 15 or more in the PPI network to identify differentially expressed genes significantly associated with prognosis

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Software

The expression of PTPN6 and its correlation analysis with survival. ( A ) Boxplot showing differential expression of PTPN6 in metastatic melanoma samples and normal skin tissue samples. ( B ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with overall survival. ( C ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with progression-free survival. ( D ) RT-qPCR assay analyzed the transcription level of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( E ) Western blotting assay analyzed the expression of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. (** p < 0.01, *** p < 0.001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: The expression of PTPN6 and its correlation analysis with survival. ( A ) Boxplot showing differential expression of PTPN6 in metastatic melanoma samples and normal skin tissue samples. ( B ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with overall survival. ( C ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with progression-free survival. ( D ) RT-qPCR assay analyzed the transcription level of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( E ) Western blotting assay analyzed the expression of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. (** p < 0.01, *** p < 0.001)

Techniques: Expressing, Quantitative Proteomics, Quantitative RT-PCR, Western Blot

Overexpression of PTPN6 inhibited the proliferation and viability of metastatic melanoma cells in vitro. ( A–C ) RT-qPCR and Western blotting assays were performed to evaluate the mRNA and protein expression of PTPN6 in A2058 and M14 cells after transfection with PTPN6. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( D , E ) CCK8 assay was used to determine the impact of PTPN6 overexpression on the proliferation of A2058 and M14 cells. ( F , G ) EdU assay was conducted to assess the impact of PTPN6 overexpression on the viability of A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as the mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 inhibited the proliferation and viability of metastatic melanoma cells in vitro. ( A–C ) RT-qPCR and Western blotting assays were performed to evaluate the mRNA and protein expression of PTPN6 in A2058 and M14 cells after transfection with PTPN6. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( D , E ) CCK8 assay was used to determine the impact of PTPN6 overexpression on the proliferation of A2058 and M14 cells. ( F , G ) EdU assay was conducted to assess the impact of PTPN6 overexpression on the viability of A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as the mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Techniques: Over Expression, In Vitro, Quantitative RT-PCR, Western Blot, Expressing, Transfection, CCK-8 Assay, EdU Assay, Standard Deviation

Overexpression of PTPN6 suppressed the migration and invasion of metastatic melanoma cells in vitro. ( A , B ) Wound healing assay was performed to determine the impact of PTPN6 overexpression on the migration of A2058 and M14 cells. ( C , D ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 overexpression on the migration and invasion of A2058 and M14 cells. ( E , F ) Western blotting assay was conducted to verify the expression of migration and invasion-related genes, including MMP2, MMP9, E-cadherin, and Vimentin, in control, NC, and PTPN6 overexpression groups in A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 suppressed the migration and invasion of metastatic melanoma cells in vitro. ( A , B ) Wound healing assay was performed to determine the impact of PTPN6 overexpression on the migration of A2058 and M14 cells. ( C , D ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 overexpression on the migration and invasion of A2058 and M14 cells. ( E , F ) Western blotting assay was conducted to verify the expression of migration and invasion-related genes, including MMP2, MMP9, E-cadherin, and Vimentin, in control, NC, and PTPN6 overexpression groups in A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Techniques: Over Expression, Migration, In Vitro, Wound Healing Assay, Western Blot, Expressing, Control, Standard Deviation

Overexpression of PTPN6 inhibited the activity of the JAK2-STAT3 signaling pathway in metastatic melanoma cells. ( A-D ) Western blotting assay was used to assess the expression of p-JAK2, JAK2, p-STAT3, and STAT3 after transfection with the PTPN6 overexpression plasmid. (**** p < 0.0001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 inhibited the activity of the JAK2-STAT3 signaling pathway in metastatic melanoma cells. ( A-D ) Western blotting assay was used to assess the expression of p-JAK2, JAK2, p-STAT3, and STAT3 after transfection with the PTPN6 overexpression plasmid. (**** p < 0.0001)

Techniques: Over Expression, Activity Assay, Western Blot, Expressing, Transfection, Plasmid Preparation

PTPN6 may affect immune cell infiltration and immune therapy response in metastatic melanoma. ( A ) TIMER 2.0 online analysis tool was used to examine the relationship between PTPN6 and the levels of infiltration of 14 immune cells and tumor purity in metastatic melanoma. ( B ) The correlation between PTPN6 and immune checkpoint therapy (alone or in combination with PD-1 or CTLA-4) response was analyzed using the TCIA database. ( C , D ) Western blotting assay was used to detect PD-L1 expression after PTPN6 overexpression (* p < 0.05, ** p < 0.01)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: PTPN6 may affect immune cell infiltration and immune therapy response in metastatic melanoma. ( A ) TIMER 2.0 online analysis tool was used to examine the relationship between PTPN6 and the levels of infiltration of 14 immune cells and tumor purity in metastatic melanoma. ( B ) The correlation between PTPN6 and immune checkpoint therapy (alone or in combination with PD-1 or CTLA-4) response was analyzed using the TCIA database. ( C , D ) Western blotting assay was used to detect PD-L1 expression after PTPN6 overexpression (* p < 0.05, ** p < 0.01)

Techniques: Western Blot, Expressing, Over Expression

Knockdown of PTPN6 promoted the proliferation, migration, and invasion of metastatic melanoma cells in vitro. ( A , B ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cells after transfection with GV341-PTPN6-3FLAG-SV40-puromycin lentivirus. ( C , D ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cell lines stably overexpressing PTPN6 after transiently transfected siPTPN6 or siNC. ( E , F ) CCK8 assay was used to determine the impact of PTPN6 knockdown on the proliferation of cells. ( G , H ) Wound healing assay was performed to determine the impact of PTPN6 knockdown on the migration of cells. ( I , J ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 knockdown on the migration and invasion of cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (** p < 0.01, *** p < 0.001, **** p < 0.0001)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Knockdown of PTPN6 promoted the proliferation, migration, and invasion of metastatic melanoma cells in vitro. ( A , B ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cells after transfection with GV341-PTPN6-3FLAG-SV40-puromycin lentivirus. ( C , D ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cell lines stably overexpressing PTPN6 after transiently transfected siPTPN6 or siNC. ( E , F ) CCK8 assay was used to determine the impact of PTPN6 knockdown on the proliferation of cells. ( G , H ) Wound healing assay was performed to determine the impact of PTPN6 knockdown on the migration of cells. ( I , J ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 knockdown on the migration and invasion of cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (** p < 0.01, *** p < 0.001, **** p < 0.0001)

Techniques: Knockdown, Migration, In Vitro, Western Blot, Expressing, Transfection, Stable Transfection, CCK-8 Assay, Wound Healing Assay, Standard Deviation

HUVEC proliferation in response to A375 or SK-Mel-28 conditioned media (C.M.). Melanoma cells were treated with 680C91 (40 µM) or epacadostat (1 µM) for 24 h, and then media were recovered, clarified and added to HUVECs. After 2 days, HUVEC proliferation was assessed. Mean ± SEM, n = 4. * p < 0.05, *** p < 0.001 vs. untreated C.M. § p < 0.05 vs. SK-Mel-28 C.M.

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: HUVEC proliferation in response to A375 or SK-Mel-28 conditioned media (C.M.). Melanoma cells were treated with 680C91 (40 µM) or epacadostat (1 µM) for 24 h, and then media were recovered, clarified and added to HUVECs. After 2 days, HUVEC proliferation was assessed. Mean ± SEM, n = 4. * p < 0.05, *** p < 0.001 vs. untreated C.M. § p < 0.05 vs. SK-Mel-28 C.M.

Article Snippet: Human metastatic melanoma cell line A375 (ATCC, Manassas, VA, USA) was grown in high D-glucose DMEM, with 10% ( v / v ) heat-inactivated fetal bovine serum (Euroclone Milan, Italy), 100 U/mL penicillin, 100 μg/mL streptomycin and 2 mmol/L glutamine in a humidified atmosphere with 5% CO 2 in air.

Techniques:

( A , B ) RT-PCR for TDO2 expression and C) Kyn release in A375 HUVECs and co-cultures. ( A ) RT-PCR in A375 alone and in A375 co-cultured with HUVECs (A375-co). Time course. Mean ± SEM, n = 5. ** p < 0.01 vs. A375 alone; ° p < 0.05, °° p < 0.01 vs. HUVEC alone; ( B ) RT-PCR in HUVECs alone and in HUVECs co-cultured with A375 (HUVEC-co). Mean ± SEM, n = 3. §§§ p < 0.001 vs. HUVECs alone; ^^ p < 0.01 vs. A375 alone. ( C ) ELISA assay for Kyn production in HUVECs, A375 and in the co-cultures (Huvec + A375) for 6, 9 and 24 h. Mean ± SEM. n = 5. * p < 0.05, ** p < 0.01 vs. HUVECs alone. The Kruskal–Wallis test and Dunn’s post hoc test were performed.

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: ( A , B ) RT-PCR for TDO2 expression and C) Kyn release in A375 HUVECs and co-cultures. ( A ) RT-PCR in A375 alone and in A375 co-cultured with HUVECs (A375-co). Time course. Mean ± SEM, n = 5. ** p < 0.01 vs. A375 alone; ° p < 0.05, °° p < 0.01 vs. HUVEC alone; ( B ) RT-PCR in HUVECs alone and in HUVECs co-cultured with A375 (HUVEC-co). Mean ± SEM, n = 3. §§§ p < 0.001 vs. HUVECs alone; ^^ p < 0.01 vs. A375 alone. ( C ) ELISA assay for Kyn production in HUVECs, A375 and in the co-cultures (Huvec + A375) for 6, 9 and 24 h. Mean ± SEM. n = 5. * p < 0.05, ** p < 0.01 vs. HUVECs alone. The Kruskal–Wallis test and Dunn’s post hoc test were performed.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

( A ) Relative mRNA expression levels of stemness markers in A375 and in A375 co-cultured for 24 h with HUVECs in the presence or absence of the TDO inhibitor. After 24 h, total RNA was extracted to perform real-time PCR. Data are shown as Mean ± SEM, n = 3. * p < 0.05 vs. A375 alone, § p < 0.05, §§ p < 0.01 vs. A375 co-cultured. ( B ) Protein expression for Myc and Klf4 in A375 and in A375 co-cultured (A375-co) for 24 h with HUVECs in the presence of the TDO inhibitor 680C91. Mean ± SEM, n = 3.

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: ( A ) Relative mRNA expression levels of stemness markers in A375 and in A375 co-cultured for 24 h with HUVECs in the presence or absence of the TDO inhibitor. After 24 h, total RNA was extracted to perform real-time PCR. Data are shown as Mean ± SEM, n = 3. * p < 0.05 vs. A375 alone, § p < 0.05, §§ p < 0.01 vs. A375 co-cultured. ( B ) Protein expression for Myc and Klf4 in A375 and in A375 co-cultured (A375-co) for 24 h with HUVECs in the presence of the TDO inhibitor 680C91. Mean ± SEM, n = 3.

Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction

Gelatin zymography for MMP2 and MMP9 activity in A375, HUVECs and in A375 co-cultured with HUVECs (co-culture) for 6–24 h. In some experiments, A375 was pretreated overnight with 680C91 or epacadostat and then co-cultures were set up. Densitometric analysis shows the effect of co-culture on latent and activated (aMMP2) MMP2 and on latent and activated (aMMP9) MMP9 at 24 h representative zymograms. Mean ± SEM of 5 experiments. § p < 0.05 vs. activated MMP9 of HUVECs; * p < 0.05, *** p < 0.001 vs. activated MMP9 of co-culture; °°° p < 0.001 vs. latent MMP9 of co-culture.

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: Gelatin zymography for MMP2 and MMP9 activity in A375, HUVECs and in A375 co-cultured with HUVECs (co-culture) for 6–24 h. In some experiments, A375 was pretreated overnight with 680C91 or epacadostat and then co-cultures were set up. Densitometric analysis shows the effect of co-culture on latent and activated (aMMP2) MMP2 and on latent and activated (aMMP9) MMP9 at 24 h representative zymograms. Mean ± SEM of 5 experiments. § p < 0.05 vs. activated MMP9 of HUVECs; * p < 0.05, *** p < 0.001 vs. activated MMP9 of co-culture; °°° p < 0.001 vs. latent MMP9 of co-culture.

Techniques: Zymography, Activity Assay, Cell Culture, Co-Culture Assay

Review

human lymph node metastatic melanoma cell line sk mel 28 (ATCC)

Structured Review

Images

Related Articles

other:

Multiple Displacement Amplification:

Similar Products

Image Search Results